Application

Phosphate buffered saline is commonly used in washing solutions for Western Blotting. It can also be used for resuspension and sonication of bacterial cells. It can be used in cell culture applications such as fixation and immunostaining.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.