Application

Phosphate Buffered Saline with 0.05% TWEEN 20 is commonly used as a wash buffer in Western blotting, enzyme linked immunosorbent assay (ELISA), dot blot, and immunohistochemistry techniques.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

TWEEN is a registered trademark of Croda International PLC

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.