Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Features and Benefits

Fast, efficient cleavage in as little as 2 hours Thrombin is covalently bound to agarose for easy removal. The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths. Cleave tags even in the presence of 0.1% Triton^™, 1 M urea or 5 mM EDTA Thrombin-agarose is reusable.

General description

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

CleanCleave is a trademark of Sigma-Aldrich Co. LLC

Quantity

200 µl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.