Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep^™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Features and Benefits

Specially formulated extraction reagents for research scale applications - save time and minimize waste Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

General description

Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Other Notes

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.