Application

Suitable for the purification of both DNA and protein from bacteria.

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

General description

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Physical form

Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.

Solution in 50% glycerol, 25 mM Sodium Acetate

Suitability

Tested for suitability with CelLytic^™ B in protein extraction from bacteria.