Application

Monoclonal ANTI-FLAG^® antibody produced in rabbit has been used in:immunofluorescencewestern blot analysisimmunocytochemistryindirect ELISABrowse additional application references in our FLAG^® Literature portal.

General description

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG^® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG^® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu²⁺ ions of the C-terminal FLAG^® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG^® peptide is found in both rat and mouse Mg²⁺ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG^® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG^® peptide sequence conjugated to KLH.

Immunogen

FLAG peptide sequence DYKDDDDK

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Provided as ascites fluid containing 15 mM sodium azide as a preservative.

Specificity

This monoclonal antibody recognizes the FLAG sequence at the N-terminus