Application

d-Desthiobiotin has been used as a component of elution buffers:to elute the precipitated proteins from the resin during coimmunoprecipitationto elute human Cdc45–MCM–GINS (CMG) helicase during chromatographic protein purificationto elute the heterodimeric kinesin family member 3A/B (KIF3A/B) and kinesin associated protein 3 (KAP3)-binding APC armadillo (APCARM) domain during chromatographic purification

d-Desthiobiotin is used in affinity chromatography and protein chromatography. d-Desthiobiotin can be used for protein labeling, detection and isolation.

Biochem/physiol Actions

Desthiobiotin is a non-sulfur containing biotin derivative that binds less strongly to biotin-binding proteins and is easily dislocated by biotin.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. D1411.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Packaging

1 g in poly bottle

500 mg in poly bottle