Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

Simple, sensitive colorimetric assay for proteins Two stable reagents are used for the working solution Faster and easier than the Lowry protein assay Compatible with many detergents both ionic and nonionic Less variation between different proteins than the Bradford dye-binding assay Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.