BM Condimed

Application

BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.

General description

BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Physiological solution, filtered through a 0.2 µm pore size membrane, buffered with sodium bicarbonate.

Preparation Note

Working concentration: 1 to 100µg/ml1 to 100µg/mlWorking solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells. Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.

Storage and Stability

Store at 2 to 8 °C. (Store in dark!)