10104159001 Display Image

ROCHE DNase Igrade II from bovine pancreas

Code: 10104159001 D2-231

Application

Isolation procedures for proteins (e.g., membrane proteins).Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-The isolation of cells from...


read more

Your Price
£106.00 EACH

Application

Isolation procedures for proteins (e.g., membrane proteins).Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-The isolation of cells from lung, skin and tumor samplesBacterial DNA extraction from live bacterial cells that are resistant to DNase I †

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].Working concentration: 1 mg/mlDo not vortex while dissolving!Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

Unit Definition

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

biological sourcebovine pancreas
formlyophilized
manufacturer/tradenameRoche
optimum pH~7.0
packagingpkg of 100 mg
Quality Level100
storage temp.2-8°C
technique(s)DNA extraction: suitable
Cas Number9003-98-9
This product has met the following criteria: