Analysis Note

Fecal coliforms appear blue, other colonies appear gray to cream colored. In some rare cases, a membrane may have confirmed fecal coliforms that are pink in color. This may be due to insufficient medium on the petri pad, excess moisture loss from Petri dish during incubation, or delayed observation of the colonies.

Target Microorganisms: Fecal coliforms

QC organismsE. coli (ATCC 25922)Mixed culture (E. coli, P. aeruginosa and P. vulgaris)

Application

m-FC Broth with rosolic acid is used to grow fecal coliforms in water and other liquid samples

Culture Medium

m-FC w/Rosolic Acid

General description

m-FC Broth with Rosolic Acid is recommended for the detection and enumeration of faecal coliforms using the membrane filter technique.{8} This medium is based on the property of faecal coliforms to grow at 44-45°C.{9} Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and minerals in m-FC Broth with Rosolic Acid. Yeast Extract is a source of vitamins and trace elements. Sodium Chloride maintains the osmotic balance. Lactose serves as a carbohydrate source. Bile Salts inhibit growth of Gram-positive bacteria. Rosolic acid is the differential indicator system. Coliforms will form blue colonies whereas non-coliforms will form gray coloured colonies.

Legal Information

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50×2 mL in ampoules

2 mL plastic ampoules

Specifications

Incubation Temperature: 44.5 °C ± 0.2

Standard Methods for the Examination of Water and Wastewater

Incubation Time: 24 hrs ± 2