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mTSB Broth with Novobiocin, for microbiology

Code: 76704-500G-F D2-231

Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products...


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£152.00 EACH
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Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

agencyaccording to ISO 16654:2001
application(s)microbiology, agriculturefood and beveragespathogen testing
colorfaint brown
compositionpeptone from soymeal, 3.0 g/L, novobiocin, 0.02 g/L, bile salts No. 3, 1.5 g/L, sodium chloride, 5.0 g/L, di-potassium hydrogen phosphate, 4.0 g/L, peptone from casein, 17.0 g/L, D(+)-glucose, 2.5 g/L
final pH7.3±0.2 (25 °C)
formpowder
manufacturer/tradenameNutriSelect® Plus
packagingpkg of -500 g
Quality Level200, 100
shelf lifelimited, expiry date on the label
sterilitynon-sterile
storage condition(Dry place, tightly sealed containers)
storage temp.2-25°C
suitabilityselective for coliforms, selective for enterovirulent E. coli (EEC), selective for Escherichia coli
technique(s)microbiological culture: suitable
This product has met the following criteria: