Analysis Note

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Application

iTRAQ^® Reagent - Multiplex Buffer Kit has been used in the isobaric tag for relative and absolute quantitation (iTRAQ) labeling of protein samples.

General description

The iTRAQ^® Reagents are a set of isobaric, multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples at the same time, allowing for simultaneous identification and quantitation, both relative and absolute, while preserving important post-translational modification (PTM) information. The iTRAQ^® reagents can be used to study the differential expression of proteins in perturbed systems.

Contains SDS – Denaturant, TCEP Reducing Reagent, MMTS Cysteine Blocking Reagent, TEAB Buffer and Isopropanol, sufficient material for either two 8-plex Multiplex Kits, three 4-plex Kits, or four mTRAQ^® 10 Assay Kits.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

MTraq is a registered trademark of AB Sciex Pte. Ltd.