Analysis Note

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Application

iTRAQ^® Reagent - 8PLEX One Assay Kit has been used in the isobaric tag for relative and absolute quantitation (iTRAQ) labeling:of digested total protein sample in mustard sproutsof the total protein in broccoli sproutsof the desalted peptides from different plants/intact chloroplasts

Features and Benefits

Simultaneously identify and quantify proteins from multiple samples Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs) Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein Increases throughput and confidence in results for protein biomarker discovery studies Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

General description

Contains iTRAQ^® Reagents 113, 114, 115, 116, 117, 118, 119 and 121, and also SDS Denaturant, TCEP Reducing Reagent, MMTS cysteine blocking reagent, TEAB buffer, and Isopropanol, for initial protocol development.This kit contains the reagents in the equivalent SCIEX iChemistry^® product number 4390811.

The iTRAQ^® reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modifications (PTM) information.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

iChemistry is a registered trademark of AB Sciex Pte. Ltd.