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Citrate Buffer, pH 6.0, 10x, Antigen Retriever,

Code: C9999-1000ML D2-231

Application

Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections p...


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£164.00 1000ML

Application

Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Other Notes

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.

Preparation Note

Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.

Storage and Stability

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

application(s)hematologyhistology
formliquid
pH6.0
Quality Level100
storage temp.2-8°C
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C9999-100ML
Unit:100ML
List Price: £76.00
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