Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.

General description

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.

Physical properties

Lyophilized powder containing PBS. Does not contain BSA as excipient

Preparation Note

Produced using animal component-free materials.

Unit Definition

One unit will reduce one µmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.