Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

General description

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Physical form

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Unit Definition

One unit will convert 1.0 µmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.