Application

β-Galactosidase from Aspergillus oryzae has been used:in in vitro nuclear magnet resonance (NMR)as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidaseto modify sugar residues on the surface of the mutant slime cells to study the adhesive propertiesfor enzymatic hydrolysis of lactosefor reversed-phase (RP) adsorptionin the hydrolysis of whey lactose

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.

General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Packaging

25000, 125000, 500000 units in poly bottle

Unit Definition

One unit will hydrolyze 1.0 µmole of lactose per minute at pH at 4.5 at 30 °C.