Isoamylase (Glycogen 6-glucanohydrolase)

Code: FSA1095 D2-0

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and analytical testing applications.

  • Isoamylase, Fructanase (FSA1085) and Amyloglucosidase (F...


     Read more

    Your Price
    £345.00 EACH
    £414.00 inc. VAT
REQUEST

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and analytical testing applications.

  • Isoamylase, Fructanase (FSA1085) and Amyloglucosidase (FSA1856, FSA1858, FSA1860) are utilised in the enzyme hydrolysis step of methods for the determination of polydextrose (a low molar mass dietary fibre) in foods
  • Refer to FSA1099 for high purity enzyme suitable for use in starch structural research

FSA1095FSA1097FSA1099
ApprovalsAOAC OMA 2000.11AOAC OMA 2000.11ISO Standard 6647-1:2015
EC Number3.2.1.68
Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry
FormulationIn 3.2M ammonium sulphate
Physical FormSuspension
CAS Number9067-73-6
Concentration~200~200~500
Synonymsisoamylase; glycogen 6-alpha-D-glucanohydrolase
SourcePseudomonas sp.Flavobacterium odoratumPseudomonas sp.
Molecular Weight715008300071500
SpecificityHydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins
Specific Activity~ 120U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16MU Sigma Units/mg)~140U/mg (40°C, pH 6.0 with 1mM CaCl2 on oyster glycogen)~240U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16MU Sigma Units/mg)
Unit DefinitionOne unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°COne unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10mg/mL) in MES buffer (100mM, pH 6.0) plus 1mM CaCl2 at 40°COne unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C
Optimal Temperature50°C
This product has met the following criteria to qualify for the following awards:



PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN
GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.