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Cellobiohydrolase I from Hypocrea jecorina, 0.13 U/mg, recombinant, expressed in corn

Code: E6412-100UN D2-231

Application

Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.

Biochem/physiol Actions

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£132.00 EACH
£158.40 inc. VAT

Application

Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.

Biochem/physiol Actions

Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles. Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.

General description

Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.

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Physical form

Provided as an ammonium sulfate precipitate with the source as recombinant maize.

Unit Definition

Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.

formliquid
greener alternative categoryEnabling
greener alternative product characteristicsDesign for Energy EfficiencyLearn more about the Principles of Green Chemistry.
Quality Level200
recombinantexpressed in corn
shipped indry ice
specific activity0.13 U/mg
storage temp.−20°C
Cas Number37329-65-0
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