Application

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:to analyse direct alkaline phosphatase (AP) activityto perform alkaline phosphatase assays with colorimetric systemto analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

General description

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP^®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)