Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: Erk (Total), Erk (Thr185/Tyr187).This is an overnight (4°C) incubation assay.This kit must be run using the provided Assay Buffer.This assay requires 25 µL diluted filtered cell lysate per well.The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).Analytes available:JNK (Total);JNK (Thr183/Tyr185)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The MILLIPLEX^® MAP Total/Phospho Akt (Catalog No. 48-622MAG) has been developed for the simultaneous detection of phosphorylated JNK (Thr183/Tyr185) and total JNK in a single well using the Luminex system.

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

96-well plate

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.