Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 µL diluted cell lysate per well.This kit must be run using Assay Buffer 1 (provided).The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).Analytes available:ATR (Total);Chk1 (Ser345);Chk2 (Thr68);H2A.X (Ser139);MDM2 (total);p21 (Total);p53 (Ser15)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The MILLIPLEX^® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: ATR (total) Chk1 (Ser345) Chk2 (Thr68) H2A.X (Ser139) MDM2 (total) p21 (total) p53 (Ser15)

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.