Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Components

Sigma’;s DirectLoad^™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Features and Benefits

Ready-to-load Easy-to-use Popular band sizes

General description

Sigma’;s DirectLoad^™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.