Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

suitable for derivatisation of Amine groups

General description

2-MercaptŒthanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

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