Application

The C-24 sequencing primer is suitable for sequencing from the C-terminus of E. coli pFLAG expression vectors.

Sequencing primers are used for double-stranded DNA sequencing of inserts in bacterial FLAG expression vectors.

Components

The primer is supplied at an initial concentration of 5 uM in 1xTE buffer. The nucleotide sequence is as follows: 5′′-OH-CTG-TAT-CAG-GCT-GAA-AAT-CTT-CTC-3′−ΟΗ

General description

C-24 Sequencing Primer is a chemically synthesized, 24 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG^® fusion proteins at the C-terminus of E. coli pFLAG expression vectors.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

(5′-CTG TAT CAG GCT GAA AAT CTT CTC-3′)