Application

The N-26 primer is used for sequencing DNA at the 5′ end of the ORF in E. coli FLAG^® expression vectors. This corresponds to the amino-terminal fusion junction of FLAG fusion proteins. It can also be used to sequence the amino-terminus of carboxy-terminal FLAG fusion proteins. Complementary sequence is immediately 5′ to the tac promoter region.

Sequencing primers are used for double-stranded DNA sequencing of inserts in bacterial FLAG expression vectors.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

(5′-CAT CAT AAC GGT TCT GGC AAA TAT TC-3′)