Shields and Sang M3 Insect Medium With L-glutamine and potassium bicarbonate. liquid sterile-filtered suitable for insect cell culture

Application

Shields and Sang M3 Insect Medium has been used to culture Drosophila cell lines, larvae, and testes.

Developed for the culture of Drosophila cell lines.

Biochem/physiol Actions

Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects. A survey of the formulas of these media reveals significant differences in their composition. When supplemented with 5-20% heat-inactivated fetal bovine serum, Shields and Sang M3 medium has been found to support the rapid growth of both primary and established cultures of Drosophila melanogaster cells.