Analysis Note

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Application

Tris base has been used:As a component of lysis buffer for cell disruptionIn the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLCIn capillary electrochromatography and UV analysis of tocopherols and tocotrienols

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

100 g in poly bottle

5 kg in poly drum

1 kg in poly bottle

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.