Application
Lipase Activity Assay Kit has been used to determine the lipase activity in samples.
Features and Benefits
Compatible with high-throughput handling systems.
General description
The lipase family of enzymes catalyze the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.
Principle
The Lipase Activity Assay kit provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in a colorimetric (570 nm) product proportional to the enzymatic activity present. One unit of Lipase is the amount of enzyme that will generate 1.0 µmole of glycerol from triglycerides per minute at 37 °C.
Suitability
Suitable for the detection of lipase activity in biological samples including tissue, cells and serum
This product has met the following criteria: