HDL and LDL/VLDL Quantitation Kit, sufficient for 100 colorimetric?�or?�fluorometric?�tests

Application

HDL and LDL/VLDL Quantitation Kit has been used to determine the concentrations of HDL and LDL/VLDL in serum samples.

General description

Lipoproteins transport the majority of plasma lipids including cholesterol and triglycerides. High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) are the lipoproteins responsible for the vast majority of cholesterol transport in the blood. High LDL levels and low HDL levels are strongly associated with increased risk of adverse cardiovascular events.

Principle

In this kit, serum HDL and LDL/VLDL are first separated and then the cholesterol concentration of each is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the cholesterol present. This kit can also be used to determine the concentration of free cholesterol and cholesteryl esters present in a sample.

Suitability

Suitable for use with plasma and serum samples.