M5057-100G Display Image

MES sodium salt, BioReagent, >=99% (titration), crystalline

Code: M5057-100G D2-231

Application

MES sodium salt has been used: To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA To equilibrate ...


read more

Your Price
£189.00 100G

Application

MES sodium salt has been used: To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization

General description

MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.

Packaging

10, 25, 100 g in poly bottle

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2µm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

application(s)diagnostic assay manufacturing
assay≥99% (titration)
formcrystalline
InChI keyIRHWMYKYLWNHTL-UHFFFAOYSA-M
InChI1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
pH8.9-10.1 (1% in water)
pKa 6.1
product lineBioReagent
Quality Level400
SMILES string[Na+].[O-]S(=O)(=O)CCN1CCOCC1
solubilitywater: 0.5 g/mL, clear, colorless
storage temp.room temp
useful pH range5.5-6.7
Code
Description
Unit Size
List Price
Qty
M5057-10G
Unit:10G
List Price: £37.20
Source:List Price
ADD
M5057-25G
Unit:25G
List Price: £59.60
Source:List Price
ADD
Cas Number71119-23-8
This product has met the following criteria:



Quantity
Est. Dispatch/Availability