M2933-25G Display Image

MES hydrate, BioPerformance Certified, suitable for cell culture, >=99.5%

Code: M2933-25G D2-231

Application

MES Hydrate has been used:To suspend cells before autophagic induction studiesIn the preparation of Murashige and Skoog growth medium for the growth of Arabid...


read more

Your Price
£51.20 25G

Application

MES Hydrate has been used:To suspend cells before autophagic induction studiesIn the preparation of Murashige and Skoog growth medium for the growth of Arabidopsis thaliana seedlingsIn the conjugation of hybridization probes to beads before PCR amplificationTo treat fibroblast-derived matrix before conjugation with heparin for use as a vascular endothelial growth factor delivery platform

General description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Other Notes

Easily compare specifications for MES products with the MES specification table.

Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.

Packaging

25, 100, 500 g in poly bottle

1 kg in poly bottle

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

application(s)diagnostic assay manufacturing
assay≥99.5%
foreign activityDNase, RNase, protease, none detected
formcrystalline powder
gradefor molecular biology, BioPerformance Certified
impuritiesendotoxin and Total Aerobic Microbial Count, tested
InChI keyMIIIXQJBDGSIKL-UHFFFAOYSA-N
InChI1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
pKa 6.1
Quality Level400
SMILES stringO.OS(=O)(=O)CCN1CCOCC1
technique(s)cell culture | mammalian: suitable
useful pH range5.5-6.7
Code
Description
Unit Size
List Price
Qty
M2933-100G
Unit:100G
List Price: £130.00
Source:List Price
ADD
M2933-500G
Unit:500G
List Price: £495.00
Source:List Price
ADD
M2933-1KG
Unit:1KG
List Price: £834.00
Source:List Price
ADD
Cas Number1266615-59-1
This product has met the following criteria:



Quantity
Est. Dispatch/Availability