Application

HEPES has been used:To supplement Dulbecco’;s modified Eagle’;s medium to culture and maintain cell linesAs a component of platelet suspension bufferTo supplement Hank′s basic salt solution, which is used to wash pancreatic tissueAs a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assayFor the adjustment and maintenance of pH of biological solutionsAs a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal developmentFor homogenization of tissue and in the preparation of cytosolic and nuclear extract from cellsAs a component of keratinocyte and fibroblast culture medium

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Packaging

100, 500 g in poly bottle

25 kg in poly drum

1 kg in poly bottle