Application

HEPES has been used:As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryoAs a component of Hanks’; solution used in the degradation testing of IronAs a component of HEPES medium in the transfection of cell culture with plasmidsAlong with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cellsAs a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri^™ Product Listing RDD002Easily compare specifications for HEPES products with the HEPES specification table.

Packaging

25, 100, 250, 500 g in poly bottle

5, 25, 50 kg in poly drum

1 kg in poly bottle