Application

Antifoam C emulsion has been used: as a supplement in RO-H₂O and a chemically defined culture medium to determinevolumetric mass transfer coefficient (k_La) of mammalian cell cultureas a component of feed medium,as asteam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivationsas a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

General description

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Other Notes

A 30% aqueous emulsion of polydimethylsiloxane concentrate.

Packaging

250, 500 mL in poly bottle