Application

HEPES has been used:As a component of SAPI medium used for the culturing of S. Typhymurium inoculumTo prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMIAs a component of dissection buffer for culturing rat cortical neuronsAlong with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapsesAs a component of Danieu′s aquarium water used in agarose gel preparation

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.