Application

Anti-GFP antibody produced in rabbit has been used in:double immunofluorescence staining immunoblot analysis.immunoprecipitation.

Biochem/physiol Actions

Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca²⁺- activated photoprotein aequorin to GFP.

Immunogen

Full length fusion protein

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN₃, 50% Glycerol.

Specificity

The antibody detects transfected proteins containing GFP tag.