Analysis Note

Positive ControlMCF7 cells

Application

Immunoblotting (2 µg/ml)r>Immunoprecipitation (2 µg)

General description

Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.

Recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~460 kDa BRCA2 protein.

Immunogen

Human

a recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST

Legal Information

Sold under license of U.S. Patents 5,837,492 and 6,124,104.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Andres, J.L., et al. 1998. Oncogene16, 2229.Chen, J., et al. 1998. Mol. Cell2, 317.Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 5287.Marmorstein, L.Y., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13869.Connor, F., et al. 1997. Nat. Genet.17, 423.Sharan, S.K., et al. 1997. Nature386, 804.Ludwig, T., et al. 1997. Genes Dev.11, 1226.Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA94, 6927.Rajan, J.V., et al. 1997. Dev. Biol.184, 385.Suzuki, A., et al. 1997. Genes Dev.11, 1242.Wong, A.K.C., et al. 1997. J. Biol. Chem.272, 31941.Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin. Oncol.23, 1.Goggins, M., et al. 1996. Cancer Res.56, 5360.Stratton, M.R. 1996. Hum. Mol. Genet.5, 1515.Tavtigian, S.V., et al. 1996. Nat. Genet.12, 333.Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA93, 13078.Spillman, M.A. and A.M. Bowcock. 1996. Oncogene13, 1639.Vaughn, J.P., et al. 1996. Cancer Res.56, 4590.Wooster, R., et al. 1995. Nature378, 789.

The CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)