Application

This Anti-Amyloid-β (oligomer) Antibody, clone F11G3 is validated for use in Western Blotting, Dot Blot, ELISA, Immunofluorescence, Immunoprecipitation for the detection of Amyloid-β.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Accumulations of insoluble deposits/aggregates are the hallmarks of several neurodegenerative diseases. Deposits involving Aβ and tau proteins in Alzheimer′s disease (AD), α-synuclein (α-syn) in Parkinson′s disease (PD), prion protein (PrP) in prion diseases (PrD), TAR DNA-binding protein 43 (TDP-43) in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar dementia-TDP (FTLD-TDP) are well known examples. Evidence suggests that oligomers represent the more toxic species than fibrillar deposits in neurodegenerative conditions, such as AD, PD, and PrD. The number of Aβ deposits, for example, correlates poorly with disease progression, and many researchers consider them to be inert or even protective. Antibodies targeting beta-sheet oligomer conformations are being developed not only to study neurotoxic oligomers, but also for diagnostic and therapeutic purposes.

Immunogen

Colloidal gold-conjugated Prp-G.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified mouse monoclonal IgMκ antibody in PBS without preservatives.

Format: Purified

Quality

Evaluated by Western Blotting of oligomeric amyloid.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected 10 µg of oligomeric amyloid.

Specificity

Target conformation is not species-specific.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Variable, depending on the sizes and the species of the oligomers formed.