Analysis Note

ControlCos-1 cell lysate

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SV40 Large T Antigen in ICC (Sabatier, J., et al. (2005). 58(4):429-431.; Del Valle, L., et al. (2004). J Virol. 78(7):3462-3469.).

Anti-SV40 Large T Antigen Antibody, clone PAb416 is an antibody against SV40 Large T Antigen for use in Western Blotting, ICC.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Immunogen

Epitope: Large T antigen of SV40

Purified large T-antigen of SV40

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Cos-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SV40 Large T Antigen in 10 µg of Cos-1 cell lysate.

Specificity

This antibody recognizes the large T antigen of SV40.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~82 kDa observed