Analysis Note

ControlL6 plus insulin cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Use Anti-Phosphoepitope SR proteins Antibody, clone 1H4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect Phosphoepitope SR proteins also known as Ser-Arg-rich proteins.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC (Fukuhara, T., et al. (2006).

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Buratti, E., et al. (2007).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including; the identification and appropriation of splice-sites, and the manipulation of alternative splicing regulation.

Immunogen

Purified oocyte nuclei containing xenopus SR proteins.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in L6 plus insulin cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected SR proteins in 10 µg of L6 plus insulin cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 75, 55, 40, 30, and 20 kDa observed.
This antibody recognizes the family of SR proteins which consist of 75, 55, 40, 30, 20 kDa proteins.