Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ago1 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot detected Ago1-containing messenager ribonucleoprotein (mRNP) complexes primarily in the low density fractions of sucrose gradient-fractionated HEK293 lysate (Höck, J., et al. (2007). EMBO Rep. 8(11):1052-1060).
Immunoprecipitation Analysis: Representative lots immunoprecipitated FLAG-tagged Ago1, but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
RNA Binding Protein Immunoprecipitation (RIP): Representative lots co-immunoprecipitated Ago1-associated RNAs, including mRNAs, miRNAs, and snoRNAs from human cell lysates (Li, Z., et al. (2009). J. Virol. 83(24):12751-12758; Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

GST-tagged recombinant human Ago1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ago1 in 10 µg of HeLa cell lysate.

Specificity

Clone 4B8 sepcifically immunoprecipitated FLAG-tagged Ago1,but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed