Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Anti-BRG1 Antibody, clone 5B7 is an antibody against BRG1/BAF190A for use in Western Blotting, Immunocytochemistry.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected BRG1/BAF190A in HeLa cell lysate.
Western Blotting Analysis: A representative lot detected BRG1/BAF190A in human HeLa, monkey Cos-1, and murine L929 cell lysates (Courtesy of Dr. Taro Tachibana, Osaka City University, Japan).
Immunocytochemistry Analysis: A representative lot detected BRG1/BAF190A in HeLa cells (Courtesy of Dr. Taro Tachibana, Osaka City University, Japan).
Immunocytochemistry Analysis: Clone 5B7 hybridoma culture supernatant detected BRIG1 co-localization with microphthalmia transcription factor (Mitf) nuclear immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized, NIH/3T3-derived B22 cells that harbors a tetracycline-inducible dominant-negative (DN) human BRG1 K798R construct regardless of DN BRG1 K798R induction by tetracycline treatment (de la Serna, I.L., et al. (2006). J. Biol. Chem. 281(29):20233-20241).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transcription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.

Immunogen

GST-tagged recombinant internal fragment from the N-terminal region of human BRG1/BAF190A.

Epitope: N-terminal region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in murine L929 fibroblast lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected BRG1/BAF190A in murine L929 fibroblast lysate.

Specificity

Expected to react with all five spliced human isoforms and both murine spliced isoforms reported by UniProt (P51532 & Q3TKT4).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~185 kDa observed. 184.6 kDa (human isoform 1), 181.3 kDa (human isoform 2), 181.6 kDa (human isoform 3), 181.7 kDa (human isoform 4), 181.3 kDa (human isoform 5), 181.4 kDa (mouse isoform 1), and 181.5 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.