Analysis Note

ControlTHP-1 cell lysate

Application

Research CategoryApoptosis & Cancer

Western Blot Analysis: A representative lot was used by an independent laboratory to detect P-Rex 1 in PC3 cell lysate (Donald, S.,et al., 2010.), bone-marrow-derived neutrophils from mice (Qin, J., et al., 2009.), and mouse brain sections. (Welch, H, et al., 2005.)

Research Sub CategoryApoptosis - Additional

This Anti-P-Rex1, clone 6F12 is validated for use in IP, WB, IH for the detection of P-Rex1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1protein (P-Rex1) is a guanine nucleotide exchange factor, which is predominantly expressed in the brain and leucocytes. It exchanges GDP for GTP in Rac 1, resulting in activation of Rac and its downstream signaling molecules that mediate such cellular processes as axon migration and differentiation.

Immunogen

Linear peptide corresponding to human P-Rex 1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in THP-1 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected P-Rex 1 in 10 µg of THP-1 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~186 kDa observed