Application

Western Blotting Analysis: A representative lot detected RKIP in Western Blotting applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).

Detects Raf kinase inhibitor protein using this mouse monoclonal Anti-RKIP, clone 7F12, Cat. No. MABC1136, tested for use in Western Blotting.

General description

Phosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 7F12 specifically detects denatured RKIP endogenously expressed in 293 cells and several pancreatic cell lines. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysates.

Specificity

Clone 7F12 specifically detects human Raf kinase inhibitor protein, (RKIP).

Target description

~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).