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Anti-IRE1alpha antibody produced in rabbit, approx. 1 mg/mL, affinity isolated antibody, buffered aqueous solution

Code: I6785-200UL D2-231


Anti-IRE1α antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

ER transmem...

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£434.00 200UL
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Anti-IRE1α antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

ER transmembrane kinase/endoribonuclease serves as an ER stress sensor that initiates the splicing of the mRNA encoding X-box–binding protein 1 (XBP-1) upon activation. Spliced XBP-1 mRNA produces homeostatic transcription factor XBP1. Under ER stress, the RNase activity of IRE1α cleaves many ER-localized mRNAs associated with apoptosis. The cytosolic motif of the activated form of IRE1α associates with the adaptor protein TRAF2 (tumor necrosis factor (TNF)–associated factor 2). This complex activates the c-Jun N-terminal kinase (JNK) signaling pathway.

Inositol-requiring enzyme-1 (IRE1) is involved in the unfolded protein response (UPR), a transcriptional program induced by endoplasmic reticulum (ER) stress. The endonuclease activity of IRE1 autoregulates its mRNA and is required for the UPR. IRE1 senses the status of luminal protein folding in the ER via its N-terminal luminal domain. Presence of unfolded and misfolded proteins leads to dimerization, trans-autophosphorylation and activation of IRE1.


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General description

The gene ERN1 (endoplasmic reticulum to nucleus signaling 1) encodes an ER transmembrane kinase/endoribonuclease that is also referred to as IRE1α (inositol-requiring transmembrane kinase and endonuclease 1α). The serine-threonine protein kinase encoded by this gene is ubiquitously expressed in cells and tissues.

Inositol-requiring enzyme-1 (IRE1), consists of an N-terminal endoplasmic reticulum (ER) luminal domain, a transmembrane domain, and a C-terminal cytoplasmic region composed of a Ser/Thr protein kinase domain and a site-specific endoribonuclease (RNase) domain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
concentration~1 mg/mL
formbuffered aqueous solution
Gene Informationhuman ... ERN1(2081)
mol wtantigen ~110 kDa
Quality Level200
shipped indry ice
species reactivityhuman
storage temp.−20°C
technique(s)indirect immunofluorescence: suitable, immunoprecipitation (IP): 2-5 µg using lysate of HEk-293T cells expressing human IRE1α, western blot: 1-2 µg/mL using whole extract of HEK-293T cells expressing human IRE1α
UniProt accession no.O75460
This product has met the following criteria: