Application

Anti-Hepatocyte Growth Factor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml is recommended. The antibody is suitable for neutralization reactions. A concentration of 500ng/ml of anti-HGF has been used to neutralize HGF in the conditioned medium of NIH3TS cells. Anti-HGF has been used at a working concentration of 20μg/ml for immunohistochemistry of section of cervical lesions.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Hepatocyte Growth Factor (HGF) is a multifunctional, heparin-binding factor that stimulates the proliferation of liver cells. The cellular effects of HGF are mediated by its receptor, c-Met, that leads to significant activation of ERK, Src/FAK, STAT3 and PI3K signaling. Cells of mesenchymal origin express HGF that stimulates the c-Met expression via an autocrine loop. Various functions of the HGF/c-Met signaling include proliferation, cell survival, motility and adhesion and tissue regeneration. Apart from liver, HGF also mediates regeneration of kidney, lung and gastro-intestines. It is also important for maintaining the insulin production and prevents hypoglycemia, self-repair of cartilage, muscle and skin and hematopoiesis. The HGF/c-Met signaling is reported as a promising therapeutic target in prevention of angiogenesis in cancer and maintaining synaptic plasticity in Alzheimer’;s disease Monoclonal Anti-Hepatocyte Growth Factor recognizes human HGF.

Immunogen

recombinant human hepatocyte growth factor.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified by Protein G.