Application

Anti-EED antibody produced in chicken is suitable for western blotting at a working dilution of 1:500 and for cell staining at a working dilution of 1:200.

Biochem/physiol Actions

The gene EED (embryonic ectoderm development) encodes a protein belonging to the Polycomb-group (PcG) family. These proteins are multimeric complexes that maintain the transcriptional repressive state of genes through successive cell generation. EED protein interacts with histone deacetylase (HDAC) proteins and mediates transcriptional repression. It forms a complex with the PRC2 histone methyltransferase complex along with EZH2 (enhancer of zeste 2) and SUZ12. This complex mediates the silencing of HOX gene expression during embryonic development. The complex plays a key role in regulating proliferation and embryogenesis. The PRC2/EED-EZH2 complex methylates ′Lys-9′ and ′Lys-27′ of histone H3 and mediates transcriptional silencing of the target genes. This complex serves as a recruiting platform for DNA methyltransferases.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Immunogen Sequence: GI # 24041020, sequence 41-168

Recombinant embryonic ectoderm development isoform a

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.