Application

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

Biochem/physiol Actions

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.

The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

Immunogen

Purified egg white avidin.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

Specificity

FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.